The Protective Effect of The Methanolic Extract of Syzgium cumini L Fruit on Kidney and Testes Tissue Damages induced by Carbon Tetrachloride

Document Type : Original Article


THE PRESENT work investigated the antioxidant effects of Syzgium cumini L fruit (jambolan) methanolic extract against Carbon tetrachloride inducing kidney and testes tissue damages in Sprague–Dawley male rats. Twenty - four adult male rats were randomly distributed into 4 groups (six rats for each).Group 1 considered as the normal control; group 2 administered with CCl4 (1.0 ml/kg body weight) in olive oil (1:1, v/v); groups 3 and 4 were treated with jambolan methanolic extract (250 and 500 mg/kg body weight, respectively) + CCl4 (1.0 ml/kg body weight). The rats were treated with limiting doses of methanolic extracts from jambolan fruit continuously for 28 days followed by an administration of CCl4 on the 27th and 28th days. Rats of the control group (group 1) received an equal volume of olive oil. At the end of the experiment biological data were calculated, blood samples were taken, kidney and testes were collected, weighted. Serum was separated to biochemical analysis. Tissue lipid peroxidation (LPX) and activity of antioxidant enzymes in kidney and testes were also performed. From the results, it could be noticed that the CCl4 may cause high serum levels in kidney functions. TBARS level also significantly increased whereas the levels of antioxidant enzymes as SOD, and CAT decreased in the kidney and testes tissue in rats treated with CCl4. Methanolic extract from jambolan fruits successfully prevented different tissues in rats. Our study demonstrated that the methanolic extract of jambolan fruits contain rich amounts of natural antioxidants which could protect kidney and testes tissues against CCl4-induced oxidative stress.